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Vesicular Stomatitis Virus

Description


vesicular stomatitis virusType: (-) ssRNA enveloped rhabdovirus

Strain: Lab-adapted Indiana strain

Tropism: The vesicular stomatitis virus envelope glycoprotein (G) mediates binding to many cellular receptors (major receptor being LDLR and LDL-like receptors) giving the virus a very broad tropism (most mammalian cell types)
Gene Map: (Arrows indicate transgene insertion sites)

vesicular stomatitis virus gene map

Proposed Uses


Standard titer stocks are ready to use in cell killing assays or for generation of new viral stocks. High-titer purified stocks are ready for immediate use in in vivo studies.

Protocol Tips


Propagation: Inoculate producer cells (e.g. Vero or BHK-21 cells) at an MOI of 0.005 for 1 hour. Collect culture supernatant when 80-90% of the cell monolayer is showing cytopathic effects (CPE, ~48 hours). Clarify the supernatant by low-speed centrifugation. Freeze and store aliquots at or below -70oC.

Viral Titration: Titer vesicular stomatitis virus by TCID50/mL endpoint dilution assay. Score CPE positive cells based on the presence of dead cells 3 days after inoculation. Alternatively, titer viruses by plaque assay and crystal violet staining. Vero or BHK-21 cells can be used for TCID50 titrations. Vero cells should not be used for titration of recombinant viruses that contain a human interferon (hIFN) transgene as Vero cells are sensitive to antiviral effects of type I IFN.

Regulatory Information


Biosafety Level: 2

US shipping license required? Yes. Please visit the USDA website to apply.

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