|Antigen||Purified stock of VSV-hIFNβ virus|
Our anti-VSV antibody is a polyclonal antibody against Vesicular Stomatitis Virus (VSV).
Recommended dilution: 1:8000 to 10,000
Western blot of VSV infected cells and VSV virions using Imanis anti-VSV Antibody
I: Total protein of VSV infected Vero cells (6 ug).
P: VSV viral stock at 8×104 TCID50 infectious units.
Anti-VSV Antibody was used at 1:8000 dilution.
Tissue: Tumor xenografts treated with VSV virotherapy (left) or saline (right)
Type: OCT cryosection
Stain: Anti-VSV antibody. Detection was with an Alexa555 fluorescent secondary antibody.
Recommended dilution: 1:1500 to 1:3000
Tissue: VSV-mIFNβ-NIS- (left) or saline- (right) treated tumor xenografts
Type: Paraffin embedded sections
Stain: Anti-VSV Antibody (REA005)
Recommended dilution: 1:5000 to 10,000
Tissue: VSV-GFP-infected Vero cells were fixed with paraformaldehyde
Type: Trypsnized cells
Stain: Pre-bleed antiserum (red) or anti-VSV antibody (blue) followed by an Alexa Fluor 594-conjugated secondary antibody
- ELISA (1:2000-1:4000 dilution to detect 1 x 107 TCID50)
- Flow cytometry analysis (1:10,000)
- Antibody neutralization (see below)
The VSV antibody has been tested for neutralization against VSV-GFP. The neutralizing titer is 2560 for complete inhibition of 500 TCID50 units of VSV-GFP on Vero cells.
Note: Users are strongly advised to determine the optimal concentration of antibody to use for their specific applications.