Imanis Life Sciences



AntigenPurified stock of VSV-hIFNβ virus
  • Description

    Our anti-VSV antibody is a polyclonal antibody against Vesicular Stomatitis Virus (VSV).

    Western Blot

    Western Blot Anti-VSV

    Recommended dilution: 1:8000 to 10,000

    Western blot of VSV infected cells and VSV virions using Imanis anti-VSV Antibody

    I: Total protein of VSV infected Vero cells (6 ug).

    P: VSV viral stock at 8×104 TCID50 infectious units.

    Anti-VSV Antibody was used at 1:8000 dilution.


    Immunofluorescence staining

    Immunofluorescence Anti-VSVTissue: Tumor xenografts treated with VSV virotherapy (left) or saline (right)

    Type: OCT cryosection

    Stain: Anti-VSV antibody. Detection was with an Alexa555 fluorescent secondary antibody.

    Dilution: 1:1500

    Recommended dilution: 1:1500 to 1:3000



    Immunohistochemistry Anti-VSV

    Tissue: VSV-mIFNβ-NIS- (left) or saline- (right) treated tumor xenografts

    Type: Paraffin embedded sections

    Stain: Anti-VSV Antibody (REA005)

    Dilution: 1:5000

    Recommended dilution: 1:5000 to 10,000


    Flow Cytometry

    Flow Cytometry Anti-VSV

    Tissue: VSV-GFP-infected Vero cells were fixed with paraformaldehyde

    Type: Trypsnized cells

    Stain: Pre-bleed antiserum (red) or anti-VSV antibody (blue) followed by an Alexa Fluor 594-conjugated secondary antibody

    Dilution: 1:10,000


    Additional Applications

    • ELISA (1:2000-1:4000 dilution to detect 1 x 107 TCID50)
    • Flow cytometry analysis (1:10,000)
    • Antibody neutralization (see below)

    The VSV antibody has been tested for neutralization against VSV-GFP. The neutralizing titer is 2560 for complete inhibition of 500 TCID50 units of VSV-GFP on Vero cells.

    Note: Users are strongly advised to determine the optimal concentration of antibody to use for their specific applications.

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