Imanis Life Sciences specializes in virological and reporter gene assays for the detection of live virus, gene transfer, gene expression, and more. We offer several standard sample analysis services:
- Replication-competent Lentivirus (RCL) Test
- Lentivirus titration (qPCR)
- In vitro sodium iodide symporter (NIS) function assays
Click below to learn more about each of our sample analysis services or to request a quote. Contact us at email@example.com for more information.
Replication-competent Lentivirus (RCL) testing
Lentiviral vector-transduced cells should be used at Biosafety level 2. However, several institutes allow in vivo experiments to be downgraded to Biosafety level 1 if the cell have been tested and found negative for replication-competent lentivirus (RCL). Imanis offers RCL testing for lentivirus using an enzyme-linked immunosorbent assay (ELISA) directed against the lentiviral p24 capsid protein. This rapid and highly-sensitive assay is the most widely-accepted test for detecting RCL. The limit of detection for our assay is 12.5 pg/mL of p24.
The cost for RCL testing is $425 per sample with bulk discounts available for order of 5 or more samples. The negative control is included in the price and is not counted as an additional sample.
Samples should be submitted as culture supernatants from cells grown for at least two weeks after lentiviral vector transduction. Culture supernatants collected earlier may yield false positive results. A negative (media only) control should also be submitted for each sample being tested.
Lentivirus titration by qPCR
Having an accurate titer for lentiviral vector stocks is important for optimizing transductions. Imanis can titer your lentiviral vectors using the same precise quantitative PCR (qPCR)-based method used for our extensive catalog of lentiviral vectors. Unlike traditional p24 ELISAs that fail to distinguish between functional and defective lentiviral particles, qPCR of integrated lentiviral genome copies exclusively measures the number of functional transducing units (TU/mL), giving you a more accurate titer to begin your studies.
Our standard qPCR titration targets the WPRE, a post-transcriptional regulatory sequence commonly included in lentiviral vectors. For titration of lentiviral vectors that do not contain a WPRE, please refer to our contract service page.
The cost for lentiviral vector titering is $425 per lentiviral vector stock. For more information or to submit samples for titration, please contact us at firstname.lastname@example.org.
NIS reporter assays
Iodine uptake assay to test sodium iodide symporter (NIS) function
Imanis provides in vitro testing to confirm NIS expression prior to in vivo delivery of viral vectors or cell lines. Samples are tested using our standard iodine (125I) uptake assay.
Pricing varies depending on the type of samples being assayed. For more information, please contact us at email@example.com
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