Cell line quality and design drive success in immuno-oncology research. At Imanis, every custom line is thoroughly characterized and quality-checked, giving you reliable tools to spend less time troubleshooting and more time generating meaningful data.
Tell Us Your Needs
At Imanis, we don’t believe in one-size-fits-all solutions, and our hands-on, collaborative approach ensures that we fully understand your scientific goals before engineering a cell line tailored to your exact needs. With deep technical expertise and a commitment to quality, we design custom cell lines that empower you to generate meaningful, reliable data and move your research forward faster.
Whether you need a reporter gene, a common TAA, or a unique transgene or mutant, Imanis engineers immortalized cell lines designed for stable, long-term expression. Using our robust lentiviral vector system, we permanently edit the host-cell genome to ensure consistent transgene stability. With flexible promoter options, including inducible systems, you gain precise control over expression to support accurate evaluation of cancer processes and therapies.
When your ideal tumor cell line needs a control that selectively reduces or ablates expression of a critical protein, Imanis delivers. Our CRISPR-engineered knock-out and knock-down monoclonal cell lines provide stable, long-lasting genomic edits, giving you powerful tools to dissect protein function with confidence.
Imanis gives you limitless flexibility when building your ideal tumor cell line. We can stack multiple modifications within the same cell line, delivering intermediate versions along the way as well as the final fully engineered product. We also create custom cell panels with clones expressing different levels of critical cancer markers, enabling more precise and informative studies.
While the exact process depends on the product you are interested in developing, here’s what you can expect during our custom cell line & vector development partnership.
Get answers to common questions about developing custom cell lines and vectors with Imanis:
Be prepared to share: the type of custom cell line you’re looking for; what the product will be used for; what it needs to do; key performance metrics; any specific technologies or reporters involved; and your timeline requirements. We use this information to generate a custom cell line proposal that you can review and confirm that the product will meet all your requirements.
Timelines vary by project complexity and type, and importantly, how quickly a particular cell line grows. During your initial consultation, we’ll discuss your timeline needs and provide a realistic delivery schedule in your custom quote.
Quality and performance start before development. With deep scientific knowledge, our team will work with you to adjust product parameters based on our experience successfully working with different cell lines and applications. If we think there is a better way to meet your research needs, we will discuss it with you. By taking time to fully understand what you need to accomplish with your custom cell line, we are able to identify key performance characteristics for the products and test them during the development and QC process.
Custom cell line products are an ideal solution when the exact combination of tumor cell line and modification isn’t available as a standard product yet. Most frequently, this happens when you have a tumor model that you have established for studies, but you need it modified to support a new project direction or develop a different therapy.
Imanis has several established methods for cell engineering. For transgene overexpression, we primarily use lentiviral vector transduction. We have a ready-to-use in-house library of lentiviral vectors encoding reporter genes and TAAs of interest, as well as lentiviral plasmids that can be used to quickly clone in new reporters of interest as needed for projects. For knock-out/knock-down, we rely on CRISPR. While not part of our typical workflows, we also have the capabilities to engineer knock-ins using CRISPR or PiggyBac to facilitate more user-specific requirements.
No. All our cell lines are research grade.
Most of the time, monoclonal cell lines are considered superior, but polyclonal cell lines can have advantages for certain applications. Polyclonal cell lines represent more of the diversity of responses typically associated with a tumor. However, this diversity can also make experiments challenging, and monoclonal lines are often preferred due to their more consistent performance between experiments. In vivo, while clones generally exhibit more reproducible growth and responses, finding the right clone for a particular application can require screening multiple clones in vivo, as in vitro growth does not always correlate to in vivo growth. Monoclonal cell lines also take longer to engineer, which can be a factor to consider if fast turnaround is needed. However, engineered polyclonal cell lines must express a selection marker to ensure the modification is present on all cells of the population, and therefore not compatible with knock-out/knock-down modifications. For overexpression lines, for ultimate flexibility, we can provide both polyclonal and monoclonal cell lines. Polyclonal cells can ship earlier to facilitate early experiments while monoclonal cell lines are being developed. For monoclonal cell lines, we generally provide 2-3 clones for each modification to facilitate clone screening and comparisons, and can even tailor clones to express various levels of transgene if requested.
Using the form on the right, tell us what you're trying to achieve, and we'll design the right solution together.
"*" indicates required fields
