A549-Fluc-Puro is a polyclonal population of the human lung carcinoma cell line A549 (ATCC® CCL-185™) .
A549-Fluc-Puro is a polyclonal population of the human lung carcinoma cell line A549 (ATCC® CCL-185™) . To achieve stable reporter expression in the polyclonal population, parental A549 cells were transduced with LV-Fluc-P2A-Puro (LV012) and selected using puromycin. LV-Fluc-P2A-Puro encodes the firefly luciferase (Fluc) cDNA linked to the puromycin resistance gene (Puro) via a P2A cleavage peptide under the spleen focus-forming virus (SFFV) promoter.
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This cell line has been tested for mycoplasma contamination and is certified mycoplasma free.
The parental A549 cell line has been authenticated and certified free of interspecies cross contamination by short tandem repeat (STR) profiling with 9 STR loci.
Low and high density cell morphology (200X)
104, 105, or 106 cells were placed in wells of a 96-well plate and 0.3 mg of d-luciferin was added to the indicated wells. The plate was immediately imaged using a Xenogen IVIS Spectrum.
107 A549-Fluc-Puro cells (Imanis Life catalog #CL080) were injected subcutaneously into the right flanks of female NCR athymic mice. Mice were imaged at day 0 on the day of cell implantation and subsequently at days 7 and 16 using a Perkin Elmer IVIS® Spectrum system, at 10-15 minutes post intraperitoneal injection of D-luciferin at 150 mg/kg. Tumor size was measured using calipers. Data from a representative mouse (ID#4878) is shown.
Complete Growth Medium: DMEM supplemented with 10% FBS, 1X Penicillin/Streptomycin, and 1 µg/mL puromycin.
The addition of puromycin to the complete growth medium maintains high reporter expression over continued passage of the cells. It is highly recommended, especially if the cells undergo multiple passages prior to being used for studies.
These cells should be grown in the indicated medium and passaged when they reach confluency. For routine passaging, cells are recommended to be split at a 1:8 ratio every 3-4 days.
These cells are suitable for in vitro and in vivo experimentation. A549 cells are a xenograph model for lung adenocarcinoma and form primary tumor and pulmonary metastases post implantation into immunosuppressed mice.1,2 The Fluc transgene facilitates in vivo noninvasive bioluminescent imaging of implanted cells.
The cells can be amplified in vitro and used to generate additional frozen stocks. Cryopreservation of low passage stocks is recommended. Frozen stocks should be preserved in a designated cryopreservation medium.
These cells were generated via lentiviral vector transduction. The lentiviral vector used for transduction was a self-inactivating (SIN) vector in which the viral enhancer and promoter have been deleted. Transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization by replication competent viruses and enables regulated expression of the genes from the internal promoters without cis-acting effects of the LTR3. Nevertheless, all work with these cells should be performed under biosafety-level 2 (BSL2) conditions by trained personnel. Institutional requirements may permit handling of these cells under BSL1 conditions if certain criteria are met.
References:
1Jiang et al. Oncogene. 2001. 20:2254-2263.
2Jenkins et al. Clin & Exp Metastasis. 2003. 20:733-744
3Miyoshi et al. J Virol 1998. 72:8150-8157.
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