Over the last 20 years, the use of lentiviral vectors to generate stable cell lines has increased dramatically. This increase is due primarily to the capacity of lentiviral vectors to mediate stable insertion of transgenes into the genome of transduced cells, facilitating stable transgene expression over many cell passages. Today, lentiviral vectors have not only […]

There are a lot of reasons to love the nuclear reporter NIS: it is non-immunogenic, gives beautiful high resolution 3D images, works for deep tissue and large animal imaging, is fully quantitative, and is translatable to the clinic. It’s an impressive list; maybe impressive enough that you want to try NIS imaging. But now what do you do? How do you get started?

Imanis Life Sciences | Rochester MN I grow a lot of reporter cell lines, and most of them are under antibiotic selection. Sure, it takes me more time to prepare culture media, but the tradeoff is that I have populations of high reporter-expressing cells that were a lot less work to make than if I […]

Imanis Life Sciences | Rochester MN It seems like there’s a lot of confusion about the immunogenicity of tumor models expressing reporter genes. In fact, the question I may get asked the most here at Imanis is probably whether our murine cell lines will grow in syngeneic immunocompetent mice. It doesn’t seem like a hard […]