How much of your lentivirus should I use for a transduction?
The optimal amount of lentivirus for transduction is cell-type dependent (e.g. primary cells require more virus than established cell lines). We recommend determining the optimal MOI for transduction by plating several wells of the target cells and infecting with increasing MOIs (e.g. 1, 3, 10, and 30*).
*For more information about calculating MOI, please see Cell Biology Protocols.