What packaging system should I use with your plasmids to generate lentivirus?
Imanis lentiviral vector transfer plasmids are meant to be used in a 3 plasmid second generation packaging system, which includes the following:
- A lentiviral transfer plasmid (from Imanis): encodes transgene and promoter
- A packaging plasmid: encode Gag, Pol, Rev, and Tat (Tat is essential for second generation packaging).
- A envelope plasmid: encoding the envelope protein (usually VSV-G).
Our transfer plasmids cannot be used with third generation packaging systems.