Imanis Life Sciences


What packaging system should I use with your plasmids to generate lentivirus?

Imanis lentiviral vector transfer plasmids are meant to be used in a 3 plasmid second generation packaging system, which includes the following:

  1. A lentiviral transfer plasmid (from Imanis): encodes transgene and promoter
  2. A packaging plasmid: encode Gag, Pol, Rev, and Tat (Tat is essential for second generation packaging).
  3. A envelope plasmid: encoding the envelope protein (usually VSV-G).

Our transfer plasmids cannot be used with third generation packaging systems.

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