Imanis Life Sciences

Menu

What packaging system should I use with your plasmids to generate lentivirus?

Imanis lentiviral vector transfer plasmids are meant to be used in a 3 plasmid second generation packaging system, which includes the following:

  1. A lentiviral transfer plasmid (from Imanis): encodes transgene and promoter
  2. A packaging plasmid: encode Gag, Pol, Rev, and Tat (Tat is essential for second generation packaging).
  3. A envelope plasmid: encoding the envelope protein (usually VSV-G).

Our transfer plasmids cannot be used with third generation packaging systems.

Learning Resources

Stay Informed

Stay up to date on the latest from the Imanis team, including product releases, promotional codes, and exciting new advancements in noninvasive in vivo imaging!