How many times can I passage your cells?

A lot. Our cells are made by lentiviral vector transduction, which means that the transgenes are stably integrated into the host cell genome and are very stable over many passages. However, keep in mind that it’s always a good idea to make a low passage stock of cells that you can go back to should any issues arise. Also, most of our cell lines are polyclonal populations, so we recommend passaging them in the presence of selecting antibiotics in order to ensure continued high reporter gene expression. The antibiotics and concentrations to use are indicated in the “Growth Conditions” tab of the relevant cell line product pages.