pLV-CAG-Luc2-P2A-Puro
Amount | 20 ug |
Full Name | pHR-SIN-CAG-Luc2-P2A-Puro |
Promoter | Synthetic hybrid CAG promoter (CMV enhancer, chicken beta-Actin promoter and rabbit beta-Globin splice acceptor site) |
Transgene | Firefly luciferase (Luc2) Puromycin resistance (Puro) |
Linker | Self-cleaving peptide P2A |
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Plasmid Map
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Validation
Luciferase Expression
HeLaH1 cells were transduced with LV-CAG-Luc2-Puro and selected with puromycin. A luciferase assay was performed and imaged with a Xenogen IVIS Spectrum.
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Proposed Use
For generation of lentiviral vector stocks encoding firefly luciferase (Luc2) under puromycin selection in a triple transfection protocol.