CT26.WT (ATCC® CRL-2638™) is a murine colorectal carcinoma cell line from a BALB/c mouse. It is a clone of the N-nitroso-N-methylrethane-induced undifferentiated CT26 colon carcinoma cell line. The cells are adherent and have a fibroblast morphology. CT26.WT cells will form tumors and metastases post implantation into syngenic BALB/c mice or immunocompromised mice.
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CT26.WT cells are suitable for in vitro and in vivo experimentation. Both syngenic BALB/c mice and immunocompromised mice can be used for in life studies, and will form tumors following implantation of the cells.
The following chart provides some examples of CT26.WT cells used for tumor formation and studies.
Hwang et al. (2021) Journal for ImmunoTherapy of Cancer 9:e002666
| Route of Implantation | Mice | Tumor/Metastases | References |
|---|---|---|---|
| Intrasplenic | BALB/c | Liver metastases | Jiao et al. (2014) J Biomed Sci 21: 1 Mi et al. (2006) J Surg Res 131: 97-104 |
| Subcutaneous | BALB/c | Subcutaneous tumor | Wang et al. (1995) J Immunol 154: 4685-4692 Hwang et al. (2021) Journal for ImmunoTherapy of Cancer 9:e002666 |
| Tail vein | BALB/c | Lung metastases | Wang et al. (1995) J Immunol 154: 4685-4692 Kim et al. (2014) Biochem Pharm 92: 251-265 Gou et al. (2014) PLoS One 9: e85789 |
| Intraperitoneal | BALB/c | Peritoneal tumor metastases | Gou et al. (2014) PLoS One 9: e85789 Ryan et al. (2015) Oncogene 34: 1563-1574 |
Our CT26.WT reporter cell lines can be tracked in vivo, making them great tools for studying the mechanisms of tumor growth and metastasis, as well as evaluating the effects of various drugs or therapies in animals. Our CT26.WT cells are available with a variety of different reporters, including the murine sodium iodide symporter (mNIS), firefly luciferase (Fluc), enhanced green fluorescent protein (eGFP), or near-infrared fluorescent protein (iRFP). Several dual reporter CT26.WT cell lines are available to facilitate multi-modality imaging.
In order to ensure high, constitutive expression of the reporter proteins, our cell lines are generated by lentiviral vector transduction. The lentiviral vectors used for these transductions are self-inactivating (SIN) vectors in which the viral enhancer and promoter has been deleted. This increases the biosafety of the lentiviral vectors by preventing mobilization of replication competent viruses (Miyoshi et al., J Virol. 1998).
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