Now Available: Multiple Myeloma Luciferase Cell Lines

Imanis Life Sciences


4T1 (Mammary Carcinoma)


4T1 (ATCC® CRL-2539™) is a murine mammary carcinoma cell line from a BALB/cfC3H mouse. The cells are adherent and have a epithelial morphology. 4T1 cells will form tumors and spontaneous metastases post implantation into syngenic BALB/c mice or immunocompromised mice, which very closely mimic stage IV human breast cancer.

*The ATCC trademark and trade name and any and all ATCC catalog numbers are trademarks of the American Type Culture Collection.

Usage Information:

4T1 cells are suitable for in vitro and in vivo experimentation. Both syngenic BALB/c mice and immunocompromised mice can be used for in life studies, and will form tumors and spontaneous metastases following implantation of the cells. Depending on the route of inoculation (see below), implanted 4T1 cells can metastasize to the lung, liver, lymph nodes, and brain, and micro-metastatic cells can be detected in many distant organs.

The following chart provides some examples of 4T1 cells used for tumor formation and studies.




Route of Implantation Mice Tumor/Metastases References
Subcutaneous BALB/c Subcutaneous tumor, lung metastases
Filatenkov et al. (2014) Radiation Res 182: 163-169.
Intracardiac BALB/c Bone metastases
Lee et al. (2014) Biochem Pharm 91: 51-60.
Zhang et al. (2012) Cancer Gen Therapy 19:630-636.
Orthotopic BALB/c Mammary tumor, Bone and lung metastases
Michigami et al. (2002) Breast Cancer Res Treat 75: 249-258.
Orthotopic Nude Mammary tumor, lung metastases
Wang et al. (2014) Biomaterials 35: 8374-8384.
Subcutaneous (mammary gland) BALB/c Mammary tumor, lung, liver, lymph node, blood, and brain metastases
Pulaski and Ostrand-Rosenberg (1998) Cancer Res 58:1486-1493.
Tail vein BALB/c Lung metastases
Thion et al. (2015) J National Cancer Institute 107
Jiang et al. (2014) Cancer Biol Ther 15: 789-796.
Intravenous BALB/c Lung metastases
Hwang et al. (2021) Journal for ImmunoTherapy of Cancer 9:e002666
Note: The above information is based on available data from the indicated references. It is not meant to be comprehensive and Imanis has not directly tested each condition.

Stable reporter cell lines:

Our 4T1 reporter cell lines can be tracked in vivo, making them great tools for studying the mechanisms of tumor growth and metastasis, as well as evaluating the effects of various drugs or therapies in animals. Our 4T1 cells are available with a variety of different reporters, including the murine sodium iodide symporter (mNIS), firefly luciferase (Fluc), enhanced green fluorescent protein (eGFP), or near-infrared fluorescent protein (iRFP). Several dual reporter 4T1 cell lines are available to facilitate multi-modality imaging.

In order to ensure high, constitutive expression of the reporter proteins, our cell lines are generated by lentiviral vector transduction. The lentiviral vectors used for these transductions are self-inactivating (SIN) vectors in which the viral enhancer and promoter has been deleted. This increases the biosafety of the lentiviral vectors by preventing mobilization of replication competent viruses (Miyoshi et al., J Virol. 1998).

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