Type: Linear dsDNA enveloped poxvirus
Tropism: Vaccinia virus enters cells via macropinocytosis and unknown cellular receptors giving the virus a broad tropism (most mammalian cell types)
Gene Map of VV(Li)-RlucGFP-hNIS-tRFP:
Standard titer stocks are ready to use for generation of new viral stocks.
Propagation: Inoculate producer cells (e.g. Vero) at an MOI of 0.1 for 2-3 hours. After 48 hours harvest culture supernatant and cells and freeze-thaw or sonicate three times. Clarify the supernatant by low-speed centrifugation and purify through a sucrose cushion. Resuspend the viral pellet in 1mM TrisCl, pH 9.
Viral Titration: Titer virus by TCID50/mL endpoint dilution assay. Score CPE positive cells based on the presence of dead cells 3-5 days after inoculation. Alternatively, titer viruses by plaque assay and crystal violet staining. Vero cells can be used for TCID50 titrations.
Risk Group: 2
USDA transport permit required? Yes, you must have a USDA transport permit to ship Vaccinia virus. Please visit the USDA website to apply.