RPMI-8226 (ATCC® CCL-155™)* cells are a multiple myeloma (MM) cell line originally isolated from the peripheral blood of a 61-year-old female plasmacytoma patient.1,2 The cell line is a protypical MM cell line. It is part of the LL-100 cell line panel, which consists of highly characterized leukemia and lymphoma cell lines for which whole-exosome and RNA sequencing is available.3,4
The RPMI-8226 cell line is considered a bona fide human myeloma cell line. It is negative for Epstein-Barr Virus, negative for CD19, expresses very low levels of CD20, but is positive for CD28 and CD38.5-7 The cells display a mature phenotype consistent with the terminal stage of B-cell differentiation. The cells express high densities of PCA1 and Ki-1, but no HLA-class II antigens and typically no B-cell antigens.8 RPMI-8226 cells produce large amounts of secreted λ-type light chains, but no γ-, α-, or μ-heavy chains or inact immunoglobulins.1
RPMI-8226 cells are widely used in immunology and immune-oncology research. Besides being useful for in vitro studies, the cells exhibit tumorigenicity in vivo in a number of immunocompromised xenograph mouse models.9-11
Our RPMI-8226 reporter cell lines can be used for in vitro or in vivo research. The luciferase (Fluc) and green fluorescence protein (GFP) reporters facilitate easy quantitation of cells for in vitro assays. Additionally, luciferase can be used for tracking tumor growth non-invasively in living animals through bioluminescence imaging. Our reporter cells are generated by lentiviral vector transduction, ensuring high, constitutive expression of the reporter proteins. The lentiviral vectors used for these transductions are self-inactivating (SIN) vectors in which the viral enhancer and promoter has been deleted. This increases the biosafety of the lentiviral vectors by preventing mobilization of replication competent viruses.12
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*The ATCC trademark and trade name and any and all ATCC catalog numbers are trademarks of the American Type Culture Collection.
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