Firefly luciferase (Fluc, Luc2), initially isolated from the firefly Photinus pyralis, is an enzyme that catalyzes the oxygenation of d-luciferin to oxyluciferin, producing visible light (530-640 nm). Several generations of Fluc have been engineered, each improving brightness. Unless otherwise specified, Imanis Fluc products have the Luc2 version of the protein (footnote), a highly optimized variant with exceptional brightness.
The bioluminescence produced by Fluc facilitates noninvasive optical imaging following the administration of d-luciferin.
Reagents: d-luciferin
Equipment: An optical imager equipped with cooled CCD cameras can be used to detect the light produced by the oxidation of d-luciferin. The acquired bioluminescent image can be overlayed with a photograph taken by the optical imager to show the relative location of the bioluminescent signal.
Additional considerations: Bioluminescence peaks about 10-12 min after the administration of d-luciferin and slowly drops.
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