pLV-SFFV-human NIS-Neo
| Amount | 20 ug |
| Full Name | pHR'-SIN-SFFV-hNIS-IRES-Neo |
| Promoter | Spleen focus-forming virus (SFFV) promoter |
| Transgene | Human sodium iodide symporter (hNIS) Neomycin resistance (Neo) |
| Linker | Internal ribosomal entry sequence (IRES) |
-
Plasmid Map
-
Validation
NIS Expression
HeLaH1 cells were transduced with LV-hNIS-Neo and selected with G418. NIS mediated I-125 uptake was assayed in the presence or absence of KClO4, a competitive inhibitor of iodine uptake. -
Proposed Use
For generation of lentiviral vector stocks encoding human sodium iodide symporter (hNIS) under G418 selection in a triple transfection protocol.