Our anti-human (ETNL) NIS antibody is a polyclonal affinity-purified antibody that detects both the native and denatured forms of the human sodium iodide symporter (hNIS). This antibody recognizes an intracellular C-terminal epitope. It does not detect rhesus, mouse, pig, nor dog NIS.

Immunofluorescence staining for human NIS expression

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Tissue: HelaH1 cells stably-expressing (left) human NIS or (right) rhesus macaque NIS

Type: Cytospin of cells on glass slide

Stain: anti-human ETNL NIS antibody followed by an Alexa Fluor 594-conjugated anti-rabbit secondary antibody and Hoechst 33342 to stain nuclei. Cell photos were taken at 200X magnification.

Dilution: 1:500 dilution

Recommended dilution: 1:500

 

Immunoblotting for human NIS protein

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Recommended dilution: 1:1000 to 1:5000

Total protein (20 ug) from parental HeLaH1 (lane 1), HeLaH1 cells stably-expressing human (lane 2), mouse (lane 3), rhesus macaque (lane 4), pig (lane 5) or dog (lane 6) NIS were subjected to SDS-PAGE and western blot analysis using anti-human ETNL NIS antibody (1:3,000 dilution) and HRP-conjugated anti-rabbit secondary antibody. A 90kDa band of NIS was detected.

For western blotting of NIS proteins, it is recommended that samples be heated at 37°C for 30 minutes prior to loading for SDS-PAGE (do not boil).

 

Flow cytometry

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Tissue: (Left panel) parental HelaH1 cells or (right panel) HelaH1 cells stably expressing human NIS linked to enhanced green fluorescent protein (GFP) were fixed and permeabilized

Type: Versenized cells

Stain: Anti-human ETNL NIS antibody followed by an Alexa Fluor-594 conjugated anti-rabbit secondary antibody

Dilution: 1:500

Recommended dilution: 1:500

Note: This antibody recognizes the cytosolic C-terminus of hNIS. Therefore, samples must be permeabilized prior to incubation with anti-human NIS antibody for immunohistochemistry and flow cytometry

Product Citations

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Wapnir et al. J.Clin Endocrinol Metab. 2003. 88:1880-1888

Li et al. FASEB J 2003. 27:3229-3238

Paroder et al. 2013. J Cell Sci 126:3305-3313

Dohan et al. Mol Endocrinol 2006. 20:1121-1137

De la Vieja et al. 2004. J Cell Sci 117:677-687

De la Vieja et al. 2005. Mol Endocrinol 19:2847-2858

Tazebay et al. 2000. Nat Med 6:871-878