Anti-hNIS antibody SJ1 is a rabbit polyclonal antibody raised against the C-terminal portion (amino acids 468-643)  of the human NIS fusion protein (MBP-hNIS).

Western Blot

Recommended dilution: 1:3000 to 1:5000

Lane 1: Total membrane protein from Mel624-hNIS-Neo cells (20 µg).

Lane 2: Total membrane protein from control murine CT26.WT-mNIS cells (20 µg). Anti-hNIS Ab was used at 1:2000 dilution. The expected size of hyperglycosylated hNIS is 75-90 kDa (top band) and hypoglycosylated hNIS is 60-65 kDa (bottom band). 

For western blotting of NIS proteins, it is recommended that samples be heated at 37°C for 30 minutes prior to loading for SDS-PAGE (do not boil).

Immunofluorescence staining

Tissue: Mel624-hNIS-Neo cells

Type: Cytospin of cells on glass slide

Stain: Secondary antibody Alexa 594 with Hoechst 33342

Dilution: 1:500

Recommended dilution: 1:500 to 1:3000

Immunohistochemical staining

Tissue: (A) human thyroid or (B) VSV-mIFNβ-NIS virus treated mouse tumor xenograft

Type: Paraffin embedded sections

Stain: Anti hNIS SJ1 antibody. Counterstain is hematoxylin.

Dilution: 1:2000

Recommended dilution: 1:2000 to 1:5000

Flow cytometry

Tissue: Human thyroid cell, SW579

Type: Versenized cells

Stain: Anti hNIS SJ1 and anti-rabbit Alexa 594

Dilution: 1:2000

Recommended dilution: 1:2000 to 1:3000

*Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.