VJ1 is a monoclonal antibody to human NIS that recognizes an epitope located between amino acid 272 and 515 located within the last three extracellular loops (shaded area).

Flow cytometry to detect human NIS

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Human NIS expression on surface of lentiviral transduced human cell lines stably expressing human NIS

Tissue: Lentiviral transduced 293 cells expressing NIS.  

Type: Fresh cells

Stain: Secondary antibody Alexa 555.

Dilution: 1:50.

Immunofluorescence staining for human NIS expression on Mel624-NIS

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Tissue: Lentiviral transduced Mel624-hNIS-Neo cells stably expressing NIS, 200x

Type: Cytospin of cells on glass slide.

Stain: Secondary antibody Alexa 555 with Hoechst 33342.

Dilution: 1:50

Immunofluorescence staining for human NIS expression on virus-infected tumor

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Tissue: Cryosection of tumor infected with an oncolytic virus expressing NIS, showing areas of NIS expressing areas, 200x.

Type: OCT cryosection .

Stain: Secondary antibody Alexa 555 with Hoechst 33342.

Dilution: 1:50

 

Note: This antibody VJ1 detects the native form of NIS and does not recognize the denatured form. The NIS monoclonal antibody clone VJ1 is suitable for flow cytometry and immunofluorescence studies of both intact and permeabilized cells

REA-IM01 (REA002)