Our anti-rat NIS antibody is a polyclonal affinity purified antibody that detects both the native and denatured forms of the rat sodium iodide symporter (rNIS). This antibody recognizes an intracellular C-terminal epitope that is conserved between rat and mouse NIS.

Immunofluorescence staining for rat NIS expression

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Tissue: HelaH1 cells stably-expressing (left) rat NIS or (right) human NIS were permeabilized

Type: Cytospin of cells on glass slide

Stain: Anti-rat NIS antibody followed by an Alexa Fluor 594-conjugated anti-rabbit secondary antibody and Hoechst 33342 to stain nuclei. Cell photos were taken at 200X magnification.

Dilution: 1:500

Recommended Dilution: 1:500

 

Immunoblotting for rat NIS protein

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Recommended Dilution: 1:1000-1:5000

Total protein (10 mg) from HeLaH1 cells stably-expressing rat (lane 1), dog (lane 2), pig (lane 3), or rhesus (lane 4) NIS were subjected to SDS-PAGE and western blot analysis using anti-rat NIS antibody (1:3,000 dilution) and HRP-conjugated anti-rabbit secondary antibody. The top band (~75-90 kDa) represents the hyperglycosylated form of rNIS, while the bottom band (~60 kDa) represents the hypoglycosylated form of rNIS. Data from Dr. Nancy Carrasco’s (Yale University) laboratory.

For immunoblotting of NIS proteins, it is recommended that samples be heated at 37°C for 30 minutes prior to loading for SDS-PAGE (do not boil).

Flow Cytometry

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Tissue: (A) HelaH1 cells stably-expressing rat NIS (blue) or parental HelaH1 cells (grey) were fixed with paraformaldehyde and permeabilized

Type: Versenized cells

Stain: Anti-rat NIS antibody followed by an Alexa Fluor 594-conjugated anti-rabbit secondary antibody

Dilution: 1:500

 

Product Citations

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Levy et al. Proc. Natl. Acad. Sci. USA. 1997. 94:5568-5573

De la Vieja et al. J. Cell Sci. 2004. 117:677-687

Nicola et al. Am. J. Physiol. Cell Physiol. 2009. 296:C654-C662

Tazebay et al. Nat. Med. 2000. 6:871-878

Paroder-Belenitsky et al. Proc. Natl. Acad. Sci. USA. 2011. 108:17933-38.