Our anti-VSV antibody is a polyclonal antibody against Vesicular Stomatitis Virus (VSV).

Western Blot

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Recommended dilution: 1:8000 to 10,000

Western blot of VSV infected cells and VSV virions using Imanis anti-VSV Antibody

I: Total protein of VSV infected Vero cells (6 ug).

P: VSV viral stock at 8×10⁴ TCID₅₀ infectious units.

Anti-VSV Antibody was used at 1:8000 dilution.

 

Immunofluorescence staining

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Tissue: Tumor xenografts treated with VSV virotherapy (left) or saline (right)

Type: OCT cryosection

Stain: Anti-VSV antibody. Detection was with an Alexa555 fluorescent secondary antibody.

Dilution: 1:1500

Recommended dilution: 1:1500 to 1:3000

 

Immunohistochemistry

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Tissue: VSV-mIFNβ-NIS- (left) or saline- (right) treated tumor xenografts

Type: Paraffin embedded sections

Stain: Anti-VSV Antibody (REA005)

Dilution: 1:5000

Recommended dilution: 1:5000 to 10,000

 

Flow Cytometry

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Tissue: VSV-GFP-infected Vero cells were fixed with paraformaldehyde

Type: Trypsnized cells

Stain: Pre-bleed antiserum (red) or anti-VSV antibody (blue) followed by an Alexa Fluor 594-conjugated secondary antibody

Dilution: 1:10,000

 

Additional Applications

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• ELISA (1:2000-1:4000 dilution to detect 1 x 10⁷ TCID₅₀)
• Flow cytometry analysis (1:10,000)
• Antibody neutralization (see below)

The VSV antibody has been tested for neutralization against VSV-GFP. The neutralizing titer is 2560 for complete inhibition of 500 TCID₅₀ units of VSV-GFP on Vero cells.

Note: Users are strongly advised to determine the optimal concentration of antibody to use for their specific applications.