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Anti-Human NIS Antibody (monoclonal, affinity purified)

Value 100 ul
Antigen Human sodium iodide symporter (hNIS)
Species Mouse
Reactivity Human
Isotype igG
  • Description

    Monoclonal antibody was raised against peptide NEDLLFFLGQKE LE corresponding to residues 598–621 of the human sodium iodide symporter (hNIS).

     

    Western blotting

    Anti-Human NIS Antibody (monoclonal, affinity purified) Western BlottingRecommended dilution: 1:1000 to 1:5000

    Membrane protein (10 μg; lanes 1-2) or total protein (50 μg; lanes 3-5) was subjected to SDS-PAGE and immunoblotting using monoclonal anti-hNIS antibody (1:3000) and HRP-conjugated anti-mouse secondary antibody (1:5000). Samples: humans NIS (lanes 1 and 4); murine NIS (lanes 2 and 5); negative control lysate (lane 3). Various glycosylated forms of human NIS are detected.

    For western blotting of NIS proteins, it is recommended that samples be heated at 37°C for 30 minutes prior to loading for SDS-PAGE (do not boil).

     

    Flow Cytometry

    Anti-Human NIS Antibody (monoclonal, affinity purified) Flow CytometryTissue: (Left) Parental HeLaH1 cells or (Right) HeLaH1 cells stably expressing hNIS linked to enhanced green fluorescent protein (GFP)

    Type: Permeabilized cells

    Stain: Anti-NIS Antibody, Alexa Fluor 594-conjugated anti-mouse secondary antibody (1: 1000)

    Dilution: 1:500

    Recommended Dilution: 1:500

     

    Immunohistochemistry

    Anti-Human NIS Antibody (monoclonal, affinity purified) Immunohistochemistry

    Tissue: Tissue sections were deparafinized. Slides were subjected to antigen retreival using 10% citrate buffer.

    a, NIS expression of normal ductal– lobular units in the vicinity of breast cancer assessed with monoclonal antibody against NIS is shown above (magnification, X160).

    b, Ductal carcinoma stained with monoclonal antibody against NIS (magnification, x66).

    Type: Paraffin Embedded Sections

    Stain: Anti hNIS Antibody (REA011)1

    Recommended Dilution: 1:5000 to 1: 10,0000

    Detection of hNIS by immunohistochemistry was performed by Tazebay et al. 2000.

    Note: This antibody recognizes the cytosolic C-terminus of hNIS. Therefore, samples must be permeabilized prior to incubation with anti-human NIS antibody for immunohistochemistry and flow cytometry

  • Citations
  • Datasheet/COA
    Lot Number
    REA-IM12

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