pLV-EF1α-humanNIS-P2A-Puro
| Amount | 20 ug |
| Full Name | pHR'-SIN-EF1α-hNIS-P2A-Puro |
| Promoter | Elongation factor-1 alpha (EF1α) promoter |
| Transgene | Human sodium iodide symporter (hNIS) Puromycin resistance (Puro) |
| Linker | Self-cleavage peptide P2A |
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Plasmid Map
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Validation
NIS Expression
HelaH1 cells were transduced with LV-EF1α-hNIS-P2A-Puro and selected with puromycin. NIS mediated I-125 uptake was assayed in the presence or absence of KClO4, a competitive inhibitor of iodine uptake. -
Proposed Use
For generation of lentiviral vector stocks encoding human NIS under puromycin selection in a triple transfection protocol.