pLV-SFFV-DRD2-PGK-Puro
| Amount | 20 ug |
| Full Name | pHR'-SIN-SFFV-DRD2-PGK-Puro |
| Promoter | Spleen focus-forming virus (SFFV) promoter Mouse phosphoglycerate kinase promoter (PGK) |
| Transgene | Human dopamine receptor D2 (DRD2) Puromycin resistance (Puro) |
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Plasmid Map
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Validation
FACS for DRD2
LV-DRD2-PGK-Puro transduced cells (red) and control cells (grey) were labeled with a spiperone derivative (DRD2 antagonist) coupled to a red fluorescent probe. After binding, cells were fixed with paraformaldehyde and analyzed by flow cytometry. -
Proposed Use
For generation of lentiviral vector stocks encoding human dopamine receptor D2 (DRD2) in a triple transfection protocol.