pLV-SFFV-eGFP-P2A-humanNIS
| Amount | 20 ug |
| Full Name | pHR'-SIN-SFFV-eGFP-P2A-hNIS |
| Promoter | Spleen focus-forming virus (SFFV) promoter Mouse phosphoglycerate kinase promoter (PGK) |
| Transgene | Enhanced green fluorescent protein (eGFP) Human sodium iodide symporter (hNIS) |
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Plasmid Map
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Validation
eGFP Expression
LV-eGFP-hNIS transduced (green) or control (grey) cells were fixed with paraformaldehyde and analyzed by flow cytometry.NIS Expression
NIS mediated I-125 uptake of LV-eGFP-hNIS transduced cells was assayed in the presence or absence of KClO4, a competitive inhibitor of iodine uptake. -
Proposed Use
For generation of lentiviral vector stocks encoding enhanced green fluorescent protein (eGFP) and human sodium iodide symporter (hNIS) in a triple transfection protocol.