pLV-SFFV-eGFP-PGK-Puro
Amount | 20 ug |
Full Name | pHR'-SIN-SFFV-eGFP-PGK-Puro |
Promoter | Spleen focus-forming virus (SFFV) promoter Mouse phosphoglycerate kinase promoter (PGK) |
Transgene | Enhanced green fluorescent protein (eGFP) Puromycin resistance (Puro) |
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Plasmid Map
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Validation
eGFP Expression
LV-eGFP-PGK-Puro transduced (green) or control (grey) cells were fixed with paraformaldehyde and analyzed by flow cytometry.
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Proposed Use
For generation of lentiviral vector stocks encoding enhanced green fluorescent protein (eGFP) under puromycin selection in a triple transfection protocol.