pLV-SFFV-hNET
Amount | 20 ug |
Full Name | pHR'-SIN-SFFV-hNET |
Promoter | Spleen focus-forming virus (SFFV) promoter |
Transgene | Human norepinephrine transporter (hNET) |
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Plasmid Map
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Validation
hNET Expression
HeLaH1 cells were transduced with LV-hNET and assayed for uptake of I-123 miBG (I-123 metaiodobenzylguanidine) as a measure of hNET activity. Parental HeLaH1 cells were used as a negative control.
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Proposed Use
For generation of lentiviral vector stocks encoding human norepinephrine transporter (hNET) in a triple transfection protocol.