pLV-SFFV-HSVTK
Amount | 20 ug |
Full Name | pHR'-SIN-SFFV-HSVTK |
Promoter | Spleen focus-forming virus (SFFV) promoter |
Transgene | Herpes simplex virus thymidine kinase (HSVTK) |
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Plasmid Map
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Validation
Thymidine Kinase Expression
HeLaH1 cells were transduced with LV-HSVTK and assayed for uptake of H-penciclovir-3 as a measure of HSVTK activity. Parental HeLaH1 cells were used as a negative control.
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Proposed Use
For generation of lentiviral vector stocks encoding herpes simplex virus thymidine kinase (HSVTK) in a triple transfection protocol.