pLV-SFFV-humanNIS-P2A-eGFP-T2A-Luc2
| Amount | 20 ug |
| Full Name | pHR'-SIN-SFFV-hNIS-P2A-eGFP-T2A-Luc2 |
| Promoter | Spleen focus-forming virus (SFFV) promoter |
| Transgene | Human sodium iodide symporter (hNIS), Enhanced green fluorescent protein (eGFP) Firefly luciferase (Luc2) |
| Linker | Self-cleavage peptide P2A and T2A |
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Plasmid Map
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Validation
NIS Expression
NIS mediated I-125 uptake of LV-hNIS-eGFP-Luc2 transduced cells was assayed in the presence or absence of KClO4, a competitive inhibitor of iodine uptake.eGFP Expression
LV-hNIS-eGFP-Luc2 transduced (green) or control (grey) cells were fixed with paraformaldehyde and analyzed by flow cytometry.Luciferase Expression
104, 105, or 106 LV-hNIS-eGFP-Luc2 transduced cells were placed in wells of a 96-well plate and 0.3 mg of d-luciferin was added to the indicated wells. The plate was immediately imaged using a Xenogen IVIS Spectrum. -
Proposed Use
For generation of lentiviral vector stocks encoding human sodium iodide symporter (hNIS), enhanced green fluorescent protein (eGFP), and firefly luciferase (Fluc) in a triple transfection protocol.