pLV-SFFV-Luc2-P2A-Puro
| Amount | 20 ug |
| Full Name | pHR'-SIN-SFFV-Luc2-P2A-Puro |
| Promoter | Spleen focus-forming virus (SFFV) promoter |
| Transgene | Firefly luciferase (Luc2) Puromycin resistance (Puro) |
| Linker | Self-cleavage peptide P2A |
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Plasmid Map
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Validation
Luciferase Expression
HeLaH1 cells were transduced with LV-Luc2-Puro and selected with puromycin. A luciferase assay was performed and imaged with a Xenogen IVIS Spectrum. -
Proposed Use
For generation of lentiviral vector stocks encoding firefly luciferase (Luc2) under puromycin selection in a triple transfection protocol.