Measles Virus
Description
Type: (-) ssRNA enveloped paramyxovirus
Strain: Edmonston vaccine lineage (live attenuated)
Tropism: The Measles virus hemagglutinin (H) protein mediates binding to the following cellular receptors
- CD46 receptor (most human tissue types)
- SLAM receptor (immune-cells)
- Nectin-4 receptor (throat epithelium)
Gene Map: (Arrows indicate transgene insertion sites)
Proposed Uses
Standard titer stocks are ready to use for generation of new viral stocks. High-titer purified stocks are ready for immediate use in in vivo studies.
Protocol Tips
Propagation: Inoculate producer cells (e.g. Vero cells) at an MOI of 0.02 for 2-3 hours. Collect the cells when 80-90% of the cell monolayer has formed syncytia. Lyse the cells by 2 freeze/thaw cycles in liquid nitrogen. Remove cell debris by centrifugation. Freeze and store aliquots at or below -70oC.
Viral Titration: Titer virus by TCID50/mL endpoint dilution assay on Vero cells. Score CPE positive cells based on the presence of syncytia 4-5 days after inoculation.
Regulatory Information
Risk Group: 2
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